ISBN: | 0367915133 (softcover : alk. paper) |
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ISBN: | 9813083352 (New York) |
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ISBN: | 7506247429 |
编目源: | DLC DLC C#P SQU CUX WSM |
个人名称: | Polak, Julia M. |
题名: | Introduction to immunocytochemistry / J.M. Polak, S. Van Noorden. |
版本说明: | 2nd ed. |
出版发行项: | Sigapore : New York : BIOS Scientific Publishers ; Springer, c1997. |
载体形态: | xiv, 141 p. : ill. ; 24 cm. |
书目附注: | Includes bibliographical references and index. |
格式化内容附注: | Introduction -- Production of antibodies -- Requirements for immunocytochemistry -- Colour plates -- Methods -- Specificity problems and essential controls -- Enhancement of standard methods -- Multiple immunostaining -- Post-embedding immunocytochemistry for the transmission electron microscope -- In vitro methods for testing antigen-antibody reactions -- Applications of immunocytochemistry -- Microscopy -- Appendix: technical notes -- Buffers -- Antibody diluent and storage of antibodies -- Adherence of preparations to slides -- Blocking endogenous peroxidase reaction -- Enzyme pre-treatment -- Heat-mediated antigen retrieval using a microwave oven -- Enzyme development methods -- Intensifying the peroxidase/DAB reaction product -- Immunostaining methods -- Immunogold staining with silver enhancement -- Double immunoenzymatic staining -- Post-embedding electron microscopical -- Absorption specificity control (liquid phase) -- References -- Index. |