书目详细信息
： Molecular cloning :
书目信息 机读格式(MARC)
| ISBN： | 7506247690 (set) |
|---|---|
| 个人名称： | Sambrook, Joseph. |
| 题名： | Molecular cloning : a laboratory manual = 分子克隆 : 实验室手册 / [Joseph Sambrook, David W. Russell]. |
| 版本说明： | 3rd ed. |
| 出版发行项： | 西安 : 世界图书出版公司, 2002. |
| 载体形态： | 3 v. : ill. ; 28 cm. |
| 书目附注： | Includes bibliographical references and index. |
| 格式化内容附注： | Vol. 1: Ch. 1. Plasmids and their usefulness in molecular cloning -- Ch. 2. Bacteriophage and its vectors -- Ch. 3. Working with bacteriophage M13 vectors -- Ch. 4. Working with high-capacity vectors -- Ch. 5. Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Ch. 6. Preparation and analysis of eukaryotic genomic DNA -- Ch. 7. Extraction, purification, and analysis of mRNA from eukaryotic cells -- Vol. 2: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction -- Ch. 9. Preparation of radiolabeled DNA and RNA probes -- Ch. 10. Working with synthetic oligonucleotide probes -- Ch. 11. Preparation of cDNA libraries and gene identification -- Ch. 12. DNA sequencing -- Ch. 13. Mutagenesis -- Ch. 14. Screening expression libraries -- Vol. 3: Ch. 15. Expression of cloned genes in Escherichia coli -- Ch. 16. Introducing cloned genes into cultured mammalian cells -- Ch. 17. Analysis of gene expression in cultured mammalian cells -- Ch. 18. Protein interaction technologies. |